effect of enzyme concentraitio essays

effect of enzyme concentraitio essays The Effect of Substrate Concentration on the Rate of Enzyme Catalase An experiment was carried out to discover if there was a relation between Hydrogen Peroxide and the enzyme Catalase. The experiment was set up as said in the method and carried out as accurately as possible. During the reaction the Hydrogen Peroxide was broken down to water and Oxygen. (H2O2 >H2O + O2). It was broken down by the enzyme catalase. A catalyst (enzyme) lower the activation energy required for the reaction to occur. Investigate how the varying concentration of Hydrogen Peroxide affects the rate of reaction. The independent variable will be the Hydrogen peroxide and the dependent variable will be the volume of gas that will be measured. Set up the equipment as it was shown in the diagram. Use the 100% Hydrogen Peroxide and to produce different concentrations of H2O2. Make up a volume of these concentrations so that the experiment can be repeated at lease twice. The concentrations are 0%, 25%, 50%, 75% and 100%. 0% = 0cm3 of H2O2 and 10cm3 of H2O 25% = 2.5cm3 of H2O2 and 7.5cm3 of H20 50% = 5cm3 of H2O2 and 5cm3 of H20 75% = 7.5cm3 of H2O2 and 2.5cm3 of H20 100% = 10cm3 of H2O2 and 0cm3 of H20 For each experiment react 1 Cm3 of Catalase with 10 cm3 of the H2O2 of different concentrations. Make sure that the measuring cylinder is completely filled with water before you begin the test. Record the volume of oxygen produced in 2 minutes. Put in a table. Calculate and convert to rate of reaction During the experiment there were no extremely dangerous chemicals used involved. But you should always make sure that the area you are working in is clear of any objects and bags. Also take care when handling glass equipment as they break easily and could harm you. ...